Scot C. Kuo, PhD

• Director of Imaging, Institute for Basic Biomedical Sciences
• Director, Microscope Facility

Associate Professor of Biomedical Engineering

• Basic Biomedical Sciences, Institute for
• Cell Dynamics, Center for

Associate Professor of Cell Biology

Actin-based protrusion and cell motility, Laser-based imaging and bioinstrumentation, Mechanical functions of cells, Nanoscale biophysics

Advanced Optics Lab - Lab Website

• The Kuo Lab is refining single-molecule detection techniques, super-resolution microscopy, multiphoton microscopy and correlative light-electron microscopy.

  By using novel optical tools, the Kuo Lab's goals are to understand cell motility and the regulation of cell shape. Regulating cell shape is important for many essential functions, including immunological defense. They have pioneered laser-based nanotechnologies, including optical tweezers, nanotracking and laser-tracking microrheology. Its applications range from physics, pharmaceutical delivery by phagocytosis (cell and tissue engineering), bacterial pathogens important in human disease and cell division.

  The Kuo lab's cell mechanics research group has created laser-based microscopy techniques to monitor the way cells change shape and exert force. They discovered molecule-sized nano-stepping of bacterial pathogens undergoing actin-based cell motility, which is a fundamental process for most cells.

  Learn more about

  • the Cell Motility & Mechanics Lab
  • how actin polymerization can push (animation); (text)

Dr. Kuo develops new technologies/methodologies and uses a multidisciplinary approach to understand the mechanical functions of cells.

As a graduate student studying bacterial chemotaxis, he used both molecular analysis of a fla operon and video-tracking hardware/software that he built to analyze switching statistics of the flagellar motor complex. As a post-doctoral fellow, he used biochemical purification/reconstitution and optical tweezers that he built, and achieved the first force measurements of the microtubule motor, kinesin, as individual molecules. As faculty, he’s built other optical instruments, including laser-deflection particle-tracking microrheology, to understand reconstituted actin-based motility and mechanics of cultured cells.

Dr. Kuo’s projects include developing instrumentation and biochemical methodology for single-molecule (TIRF) and super-resolution (PALM) imaging of reconstituted actin-based motility.

http://scholar.google.com/citations?user=_9FtmK8AAAAJ&hl=en&oi=ao

http://www.ncbi.nlm.nih.gov/pubmed?Db=pubmed&Cmd=Search&Term=Scot+Kuo%5BAuthor%5D

• Cockburn IA, Amino R, Kelemen RK, Kuo SC, Tse SW, Radtke A, Mac-Daniel L, Ganusov VV, Zavala F, Ménard R. “In vivo imaging of CD8+ T cell-mediated elimination of malaria liver stages.” Proc Natl Acad Sci U S A. 2013 May 28;110(22):9090-5. doi: 10.1073/pnas.1303858110

• DeRose R, Pohlmeyer C, Umeda N, Ueno T, Nagano T, Kuo S, Inoue T. “Spatio-temporal manipulation of small GTPase activity at subcellular level and on timescale of seconds in living cells.” J Vis Exp. 2012 Mar 9;(61). pii: 3794. doi: 10.3791/3794.

• Fisher CI, Kuo SC. “Filament rigidity causes F-actin depletion from nonbinding surfaces.” Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):133-8. doi: 10.1073/pnas.0804991106.

• Reichl EM, Ren Y, Morphew MK, Delannoy M, Effler JC, Girard KD, Divi S, Iglesias PA, Kuo SC, Robinson DN. “Interactions between myosin and actin crosslinkers control cytokinesis contractility dynamics and mechanics.” Curr Biol. 2008 Apr 8;18(7):471-80. doi: 10.1016/j.cub.2008.02.056.

• Yamanaka S, Campbell NR, An F, Kuo SC, Potter JJ, Mezey E, Maitra A, Selaru FM. “Coordinated effects of microRNA-494 induce G?/M arrest in human cholangiocarcinoma.”Cell Cycle. 2012 Jul 15;11(14):2729-38. doi: 10.4161/cc.21105.

• Plenary speaker, 1/1/05
• Robert B. Pond Sr. Excellence in Teaching Award, Johns Hopkins University, 1/1/01
• Keynote speaker, Annual Meeting of American Society for Biomechanics, 1/1/91
• Fellow, Jane Coffin Childs Memorial Fund for Medical Research, 1/1/89
• Phi Beta Kappa, Harvard University chapter, 1/1/82
• Predoctoral fellow, National Science Foundation, 1/1/82
• Magna cum laude with Highest Honors in Biochemistry, 1/1/82

• American Society for Cell Biology
• Biomedical Engineering Society
• Biophysical Society

• American Journal of Physiology, Reviewer
• Biophysical Journal, Reviewer
• Journal of Biological Chemistry, Reviewer
• Journal of Cell Biology, Reviewer
• National Institutes of Health, Ad hoc reviewer, 1/1/96
• National Science Foundation, Special reviewer, 1/1/92
• Nature, Reviewer
• Nature Cell Biology, Reviewer
• Science, Reviewer

Duke Medical Center, Durham, NC, 1993, Cell Biology; Washington University, St. Louis, MO, 1991, Cell Biology; University of California, Berkeley, CA, 1988