Kristine Glunde, MS, PhD

Director, Applied Imaging Mass Spectrometry Core

Professor of Radiology and Radiological Science

Sidney Kimmel Comprehensive Cancer Center

https://www.linkedin.com/in/kristine-glunde-468a114/

Professor of Oncology, Professor of Pathology

Cancer, Breast Cancer, Metabolism, Choline, Phospholipid, Creatine, Glutamine, Magnetic Resonance Spectroscopic Imaging, Mass Spectrometry Imaging, Multimodal, Molecular, Cellular. Imaging, Fluorescence

Kristine Glunde Lab - Lab Website

• Dr. Glunde's Research Program is within the Division of Cancer Imaging Research in the Department of Radiology and Radiological Science and focuses on cancer biology and molecular imaging of cancer. Her lab combines cancer biology approaches with multi-scale molecular imaging to investigate and visualize molecular events that drive cancer growth, invasion, and metastasis. Her lab also develops novel applications which integrate mass spectrometry imaging with optical microscopy technologies. Molecular imaging technologies used in Dr. Glunde's lab span magnetic resonance imaging, mass spectrometry imaging, and fluorescence imaging.

Applied Imaging Mass Spectrometry - AIMS Core / Service Center, iLab

Applied Imaging Mass Spectrometry – AIMS Core/Service Center

Dr. Glunde is Professor of Radiology, Oncology and Biological Chemistry at The Johns Hopkins University School of Medicine, and the founding Director of the Applied Imaging Mass Spectrometry (AIMS) Core. Her research program focuses on cancer biology and molecular imaging of cancer. Her lab combines cancer biology approaches with multi-scale molecular imaging to investigate and visualize molecular events that drive cancer growth, invasion, and metastasis. Her lab also develops novel applications which integrate mass spectrometry imaging with optical microscopy technologies. Molecular imaging technologies used in Dr. Glunde's lab span magnetic resonance imaging, mass spectrometry imaging, and fluorescence imaging.

https://scholar.google.com/citations?user=OmK7fxcAAAAJ&hl=en

https://pubmed.ncbi.nlm.nih.gov/?term=Glunde+K&sort=date

1. Jenkinson NM, Oza H, Yarena KJ, Glunde K*. Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells. Sci. Rep. 2026; 16: 20021. doi: 10.1038/s41598-026-38920.      
2. Hahm TH, Brown DR, Tressler CM, Tran T, Ly A, Pathak AP, McMahon MT, Glunde K*. Quantitative MALDI imaging of aspirin metabolites in mouse models of triple-negative breast cancer. Theranostics 2025; 15: 9359-9374. doi: 10.7150/thno.116819.
3. Yang E, Kim JH, Tressler CM, Shen XE, Brown DR, Johnson CC, Hahm TH, Barman I*, Glunde K*. RaMALDI: enabling simultaneous Raman and MALDI imaging of the same tissue section. Biosens. Bioelectron. 2023; 239: 115597.
4. Yang E, Shen XE, West-Foyle H, Hahm TH, Brown DR, Johnson CC, Kim JH, Roker LA, Tressler CM, Barman I, Kuo SC, Glunde K*. FluoMALDI microscopy: matrix co-crystallization simultaneously enhances fluorescence and MALDI imaging. Adv. Sci., 2023; e2304343. doi: 10.1002/advs.202304343.
5. Tan Z, Boyapati K, Tressler CM, Jenkinson J, Glunde K*. Glutamine transporter SLC38A3 promotes breast cancer metastasis via Gsk3ß/ß-catenin/EMT pathway. Cancer Lett. 2024; 586: 216653. doi: 10.1016/j.canlet.2024.216653.

Mass Spectrometry Imaging - Focus on MALDI Imaging

1. Fluorescent 6’-O-glucosamine compounds to label lysosomes in tumors in vivo and in cell culture systems for detection with optical imaging or fluorescence microscopy. Glunde K, Li C, Bhujwalla ZM. U.S.Provisional Patent JHU reference number: 4739, 5/31/05
2. Near infrared fluorescent 6-O-glucosamine compounds with targeting specificity to lysosomes in tumors. Glunde K, Foss CA, Bhujwalla ZM. U.S. Provisional Patent JHU reference number: 4739, 7/13/06
3. Enhanced fluorescence signal through the application of aromatic additives onto the microscopy sample for standard fluorescence, fluorescence microscopy and combined fluorescence MALDI microscopy/imaging. Yang E, Tressler C, Kuo SC, West-Foyle HK, Shen XE, Glunde K. PCT/US2023/019162. US Patent App. 18/854,736, 4/19/23
4. Improved Raman spectroscopy and combined Raman-matrix-assisted laser desorption/ionization (MALDI) imaging workflow with the addition of aromatic compounds. Yang E, Kim JH, Tressler C, Barman I, Glunde K.  PCT/US2023/16943, 4/19/23

• ISMRM (International Society for Magnetic Resonance in Medicine) Senior Fellowship Award, 2024
• Radiology Britestar Award, Johns Hopkins University School of Medicine, 2017
• Delegate at 57th Meeting of Nobel Laureates in Physiology or Medicine in Lindau, Germany, 2007
• 1st Place Poster Award MR Spectroscopy – ISMRM in Miami, Florida, USA, 2005
• Career Development Award, In Vivo Cellular and Molecular Imaging Center, Johns Hopkins University School of Medicine, 2003
• FNK Research Foundation Ph.D. Student Stipend, University of Bremen, 1997-2000
• Excellence Award “Stiftung Constancia von 1823” for the Diploma Thesis, University of Bremen, 1997

Cellular and Molecular Medicine

• American Society for Mass Spectrometry (ASMS), 2005-Present
• World Molecular Imaging Society (WMIS), 2004-Present
• American Society for Cell Biology (ASCB), 2003-2008
• American Association for Cancer Research (AACR), 2002-Present
• International Society for Magnetic Resonance in Medicine (ISMRM), 1999-Present

Positions and Scientific Appointments

• Co-Director, Mass Spectrometry Molecular Imaging and Multi-Omics Shared Resource, The Johns Hopkins Sidney Kimmel Comprehensive Cancer Center, JHU SOM, Baltimore, MD, 2021-present
• Professor, Joint Appointment, Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 2021-2025
• External Advisory Board for Small Animal Imaging Facility, University of Pennsylvania, PA, 2020-Present
• Founding Director, Applied Imaging Mass Spectrometry Core, 2019-Present
• Board of Trustees, International Society for Magnetic Resonance in Medicine, 2018-2021
• Search Committee, Search for Director of Biological Chemistry Department, 2018-2019
• Planning Committee, Space Renovation, Applied Imaging Mass Spectrometry (AIMS) Core, Cancer Research Building II, Room LB03, 2018-2019
• External Advisory Board for NIH P41EB015908, National Center for In Vivo Metabolism, UT Southwestern, Dallas, TX, 2018-2023
• Career Development Advisory Committee (CDAC), Radiology Department, 2017-Present
• Professor, Departments of Radiology and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, 2017-Present
• Elected Chair, Magnetic Resonance in Cancer Study Group, International Society of Magnetic Resonance of Medicine, 2012-2013
• Program Committee, World Molecular Imaging Congress, 2012-2013
• Program Committee, International Society for Magnetic Resonance in Medicine, 2011-2015
• Associate Professor, Departments of Radiology and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, 2009-2017
• Oversight Committee, School of Medicine Radiology Office of Research Administration (RORA), Radiology Department, Johns Hopkins Medical Institutions, 2008-Present
• Co-organization of CME-accredited JHU ICMIC Seminar Series, Johns Hopkins Medical Institutions, 2005-Present
• Assistant Professor, Departments of Radiology and Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, 2005-2009
• Oversight Committee, Molecular Imaging Service Center, Johns Hopkins Medical Institutions. 2004-Present
• Joint Appointment in Department of Oncology, SKCCC, Johns Hopkins University School of Medicine, Baltimore, MD, 2004-Present
• Instructor, Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 2003-2005
• Postdoctoral Fellow, Department of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 2000-2003

Grant Reviewer

• Co-Chair IMAT Study Section, 2021-2022
• Charter Member, Reviewer for MEDI, Medical Imaging Study Section, NIH, 2014-2018
• Ad Hoc Reviewer IMAT Study Section, and various other NIH review panels, 2012-Present
• Reviewer for the California Breast Cancer Research Program (CBCRP), 2011
• Reviewer, Breast Cancer Research Program, Department of Defense, Congressionally Directed Medical Research Programs. 2009-2012

Editorial Boards

• Editorial Board, Section Editor, Biomarkers for the Molecular Imaging and Biology (MIBI), 2020-2023
• Editorial Board, Section Editor, Cancer Imaging and Image-directed Interventions, Frontiers in Oncology, 2015-Present
• Editorial Board, American Journal of Nuclear Medicine and Molecular Imaging (AJNMMI), 2013-Present
• Editorial Board, Journal ‘Biomedical Spectroscopy and Imaging’, IOS Press, 2012-Present

Johns Hopkins University, Baltimore, MD, 2003